• Product NameCollagen IV α5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Collagen IV α5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen IV α5.
• Target NameCollagen IV α5
• Alternative Names    Collagen alpha-5(IV) chain ; CO4A5;
• Accession No. Swiss-Prot: P29400
  NCBI Gene ID: 1287
• UniprotP29400
• Gene ID1287;
• SDS-PAGE MW 161kd
• Concentration 1.0mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage Store at -20˚C

• IHC 1:100-1:300
  IF 1:200-1:1000

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Zhou J., J. Biol. Chem. 269:6608-6614(1994).
Guo C., Kidney Int. 44:1316-1321(1993).
Nakazato H., Kidney Int. 46:1307-1314(1994).

• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Collagen IV α5 antibody #34204.
• Immunofluorescence analysis of HeLa cells, using Collagen IV α5 antibody #34204.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting