• Product NameCollagen VI α3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Collagen VI α3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen VI α3.
• Target NameCollagen VI α3
• Alternative Names    Collagen alpha-3(VI) chain; CO6A3;
• Accession No. Swiss-Prot: P12111
  NCBI Gene ID: 1293
• UniprotP12111
• Gene ID1293;
• SDS-PAGE MW 344kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Collagen VI acts as a cell-binding protein.

Chu M.-L., EMBO J. 9:385-393(1990).
Zanussi S., J. Biol. Chem. 267:24082-24089(1992).
Liu T., J. Proteome Res. 4:2070-2080(2005).

• mmunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Collagen VI α3 antibody #34209. The picture on the right is blocked with the synthesized peptide.
• Immunofluorescence analysis of HeLa cells, using Collagen VI α3 antibody #34209. The picture on the right is blocked with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting