• Product NameCTXB beta subunit Cholera Toxin Antibody Cy3 Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsICC IF
• Species ReactivityCholera Toxin
• Crossing ReactivityCholera Toxin Subunit B
• Immunogen DescriptionKLH conjugated synthetic peptide derived from CTXB beta subunit Cholera Toxin
• ConjugatesCy3
• Excitation Emission512,550nm 570,615nm
• Target NameCTXB beta subunit Cholera Toxin
• Alternative Names    Cholera enterotoxin B chain; Cholera enterotoxin beta chain; Cholera enterotoxin gamma chain; Cholera enterotoxin subunit B; Cholera toxin B protein; Choleragenoid; CTX B; CTXB; TOX B; TOXB; VC1456; CHTB_VIBCH.
• Accession No. NCBI Gene ID:2613962
• Gene ID2613962
• Concentration 1mg/ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• ICC=1:50-200 IF=1:50-200

The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting