• Product NameAML1(RUNX1) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total AML1(RUNX1) protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.6~10(D-A-S-T-S) derived from Human AML1(RUNX1).
• Target NameAML1(RUNX1)
• Alternative Names    CBFA2; EVI-1; AMLCR1
• Accession No. Swiss-Prot: Q01196
  NCBI Protein: NP_001116079.1
• UniprotQ01196
• Gene ID861;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 55kd
  Western blotting: 1:500~1:1000

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The a subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.Mol. Cell. Biol. 19:3635-3644(1999)
Pelletier N., Champagne N., Stifani S., Yang X.-J. Oncogene 21:2729-2740(2002)
Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A.J. Biol. Chem. 279:19512-19522(2004)

• Western blot analysis of extract from P19, Hela and 293 cells using AML1(RUNX1) Antibody #21477
• Western blotting analysis using AML1(RUNX1) Antibody #21477.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting