• Product NameLPAM-1(Integrin a4, CD49d) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total LPAM-1(Integrin a4, CD49d) protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.1024~1028(Y-I-N-S-K) derived from Human LPAM-1(Integrin a4, CD49d).
• Target NameLPAM-1(Integrin a4,CD49d)
• Alternative Names    IA4; MGC90518; ITGA4
• Accession No. Swiss-Prot: P13612
  NCBI Protein: NP_000876.3
• UniprotP13612
• Gene ID3676;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 70 140 180kd
  Western blotting: 1:500~1:1000
  

Integrins a-4/beta-1 (VLA-4) and a-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin a-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin a-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Iida J., Meijne A.M.L., Oegema T.R. Jr. J. Biol. Chem. 273:5955-5962(1998)
Liu S., Thomas S.M., Woodside D.G., Rose D.M. Nature 402:676-681(1999)

• Western blot analysis of extract from JK and Raji cells using LPAM-1(Integrin a4, CD49d) Antibody #21616

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting