• Product NameGFAP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous level of total GFAP protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 423~427 (E-S-K-Q-E) derived from Human GFAP.
• Target NameGFAP
• Alternative Names    FLJ45472
• Accession No. Swiss-Prot: P14136
  NCBI Protein: NP_002046.1
• UniprotP14136
• Gene ID2670;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 50kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Bongcam-Rudloff E., Nister M., Betsholtz C., Wang J.-L., Stenman G., Huebner K., Croce C.M., Westermark B.Cancer Res. 51:1553-1560(1991) Nielsen A.L., Holm I.E., Johansen M., Bonven B., Jorgensen P., Jorgensen A.L.J. Biol. Chem. 277:29983-29991(2002)

• Western blot analysis of extract from Rat brain, Rat hippocampus tissue and C6 cells using GFAP Antibody #21485
• Immunofluorescence staining of paraffin-embedded Glioma cells of Spinal Cord using GFAP Antibody #21485.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting