• Product NameWASP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total WASP protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.289~293 (L-I-Y-D-F) derived from Human WASP.
• Target NameWASP
• Alternative Names    THC; THC1; IMD2
• Accession No. Swiss-Prot: P42768
  NCBI Protein: NP_000368.1
• UniprotP42768
• Gene ID7454;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 53kd
  Western blotting: 1:500~1:1000
  

Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.

Kolluri R., Tolias K.F., Carpenter C.L., Rosen F.S. Proc. Natl. Acad. Sci. U.S.A. 93:5615-5618(1996)
Ramesh N., Anton I.M., Hartwig J.H., Geha R.S. Proc. Natl. Acad. Sci. U.S.A. 94:14671-14676(1997)
Cory G.O.C., Cramer R., Blanchoin L., Ridley A.J. Mol. Cell 11:1229-1239(2003)

• Western blot analysis of extract from Hela, JK and Raji cells using WASP Antibody #21632

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting