• Product NameMyc Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total POLE1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human POLE1.
• Target NameMyc
• Alternative Names    DNA polymerase epsilon catalytic subunit A; EC 2.7.7.7; DNA polymerase II subunit A;
• Accession No. Swiss-Prot: Q07864
  NCBI Gene ID: 5426
• UniprotQ07864
• Gene ID5426;
• SDS-PAGE MW 261kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  

Participates in DNA repair and in chromosomal DNA replication.

Kesti T., J. Biol. Chem. 268:10238-10245(1993).
Syvaeoja J.E.;Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Asahara H., Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using POLE1 antibody #34647.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting