• Product NameEPHA10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified by immunogen affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHuman Mouse Rat
• SpecificityThe antibody detects endogenous levels of total NEIL3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide of human NEIL3.
• Target NameEPHA10
• Alternative Names    Endonuclease VIII-like 3; Nei-like 3; DNA glycosylase FPG2;
• Accession No. Swiss-Prot: Q8TAT5
  NCBI Gene ID: 55247
• UniprotQ8TAT5
• Gene ID55247;
• SDS-PAGE MW 68kd
• Concentration 1.0mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.

Takao M., J. Biol. Chem. 277:42205-42213(2002).
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

• Western blot analysis of NEIL3 expression in MCF7 (A), H1792 (B), rat thymus (C), mouse testis (D) whole cell lysates.
• Immunohistochemical analysis of NEIL3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting