• Product NameC-Fos Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total C-Fos protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.28~32( S-Y-Y-H-S) derived from Human C-Fos.
• Target NameC-Fos
• Alternative Names    p55; AP-1;
• Accession No. Swiss-Prot: P01100
  NCBI Protein: NP_005243.1
• UniprotP01100
• Gene ID2353;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 62kd
  Western blotting: 1:500~1:1000
  

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

Okazaki K., Sagata N.EMBO J. 14:5048-5059(1995)
Zhang Y., Feng X.H., Derynck R.Nature 394:909-913(1998)
Bossis G., Malnou C.E., Farras R. Mol. Cell. Biol. 25:6964-6979(2005)

• Western blot analysis of extracts from A549, Hela and 3T3 cells using C-Fos Antibody #21667.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting