• Product NamePPARg Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PPARg protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.50~54 (L-S-V-M-E) derived from Human PPARg
• Target NamePPARg
• Alternative Names    GLM1; NR1C3; PPARG1; PPARG2; PPARG
• Accession No. Swiss-Prot: P37231
  NCBI Protein: NP_005028.4
• UniprotP37231
• Gene ID5468;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 53 57kd
  Western blotting: 1:500~1:1000
  

Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. "Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARgamma2 versus PPARgamma1 and activation with retinoid X receptor agonists and antagonists."

Mukherjee R., Jow L., Croston G.E., et al. J. Biol. Chem. 272:8071-8076(1997) "Cloning and characterization of RAP250, a nuclear receptor coactivator."
Caira F., Antonson P., Pelto-Huikko M., et al. J. Biol. Chem. 275:5308-5317(2000) "Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor a."
Qi C., Chang J., Zhu Y.,et al. J. Biol. Chem. 277:28624-28630(2002)

• Western blot analysis of extracts from Hela, 293, 3T3 and MCF-7 cells using PPARg Antibody #21649.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting