• Product NameNOX1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NOX1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human NOX1.
• Target NameNOX1
• Alternative Names    NADPH oxidase 1; NOX-1; NOH-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; Mitogenic oxidase 1
• Accession No. Swiss-Prot: Q9Y5S8
  NCBI Gene ID: 27035
• UniprotQ9Y5S8
• Gene ID27035;
• SDS-PAGE MW 65kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

NOH-1S is a voltage-gated proton channel that mediates the H+ currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H+ ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.

Suh Y.-A., Nature 401:79-82(1999).
Banfi B., Science 287:138-142(2000).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from 293 cells, using NOX1 antibody #34844.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting