STAT1(Phospho-Tyr701) Antibody - SAB

Catalog No.	Package Size	Price	Quantity
11044-1	50ul	$219.00
11044-2	100ul	$319.00

Catalog No.

Package Size

Price

Quantity

11044-1

50ul

$219.00

11044-2

100ul

$319.00

Product NameSTAT1(Phospho-Tyr701) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1.
Target NameSTAT1
ModificationPhospho
Alternative Names CANDF7, ISGF-3, STAT91

Accession No. Swiss-Prot: P42224
NCBI Protein: NP _009330.1
UniprotP42224
Gene ID6772;
SDS-PAGE MW 84,91kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 84,91kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Durbin J E, et al. (1996) Cell. 84: 443-450.
Meraz M A, et al. (1996) Cell. 84: 431-442.
Wakao H, et al. (1994) EMBO J. 13: 2182-2191.
Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258
Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.

STAT1(Phospho-Tyr701) Antibody #11044

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