• Product NameKeratin 7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Keratin 7 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.22~26(A-Q-V-R-L) derived from Human Keratin 7
• Target NameKeratin 7
• Alternative Names    K7; CK7; SCL; K2C7; MGC3625
• Accession No. Swiss-Prot: P08729
  NCBI Protein: NP_005547.3
• UniprotP08729
• Gene ID3855;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 52kd
  Western blotting: 1:500~1:1000
  

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). "Sarcolectin (SCL): structure and expression of the recombinant molecule."

Kaba A., Jiang P., Chany-Fournier F. Biochimie 81:709-715(1999) "Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7."
Lin L., Holbro T., Alonso G. J. Cell. Biochem. 80:483-490(2001) "Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit a(1)-globin and human cytokeratin 7."
Kanduc D. J. Virol. 76:7040-7048(2002)

• Western blot analysis of extracts from Hela cells using Keratin 7 Antibody #21648.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting