• Product NameARHGEF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ARHGEF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ARHGEF1.
• Target NameARHGEF1
• Alternative Names    p115-RhoGEF; p115RhoGEF; 115 kDa guanine nucleotide exchange factor; Sub1.5;
• Accession No. Swiss-Prot: Q92888
  NCBI Gene ID: 9138
• UniprotQ92888
• Gene ID9138;
• SDS-PAGE MW 105kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.

Farmer A., Submitted (MAY-2003).
Wedegaertner P.B., J. Biol. Chem. 275:14992-14999(2000).
Han D.K., Sci. Signal. 2:RA46-RA46(2009).

• Western blot analysis of extracts from COLO205, HepG2, HuvEc and Jurkat cells, using ARHGEF1 antibody #34990.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting