• Product NameARHGEF12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ARHGEF12 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ARHGEF12.
• Target NameARHGEF12
• Alternative Names    ARHGC; ARHGEF12; Leukemia-associated RhoGEF; RHO guanine nucleotide exchange factor (GEF) 12 (LARG); Rho guanine nucleotide exchange factor 12
• Accession No. Swiss-Prot: Q9NZN5
  NCBI Gene ID: 23365
• UniprotQ9NZN5
• Gene ID23365;
• SDS-PAGE MW 173kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.

Kourlas P.J., Proc. Natl. Acad. Sci. U.S.A. 97:2145-2150(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Nagase T., DNA Res. 4:141-150(1997).

• Western blot analysis of extracts from RAW264.7 cells, using ARHGEF12 antibody #34992.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting