• Product NameE2F-1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total E2F-1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.430~434(G-D-L-T-P) derived from Human E2F-1
• Target NameE2F-1
• Alternative Names    RBP3; E2F-1; RBAP1; RBBP3;
• Accession No. Swiss-Prot: Q01094
  NCBI Protein: NP_005216.1
• UniprotQ01094
• Gene ID1869;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 70kd
  Western blotting: 1:500~1:1000
   

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. "P53 and E2F-1 cooperate to mediate apoptosis."

Wu X., Levine A.J. Proc. Natl. Acad. Sci. U.S.A. 91:3602-3606(1994) "Regulation of E2F1 activity by acetylation."
Martinez-Balbas M.A., Bauer U.M., Nielsen S.J., Brehm A., Kouzarides T. EMBO J. 19:662-671(2000) "Regulation of E2F1 function by the nuclear corepressor KAP1."
Wang C., Rauscher F.J. III, Cress W.D., Chen J. J. Biol. Chem. 282:29902-29909(2007)

• Western blot analysis of extracts from 3T3 cells using E2F-1 Antibody #21646.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting