• Product NameHDAC3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total HDAC3 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.420~424(N-D-K-E-S) derived from Human HDAC3.
• Target NameHDAC3
• Alternative Names    HD3; RPD3; RPD3-2
• Accession No. Swiss-Prot: O15379
  NCBI Protein: NP_003874.2
• UniprotO15379
• Gene ID8841;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 49kd
  Western blotting: 1:500~1:1000
   

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation

Wei L.-N., Hu X., Chandra D., Seto E., Farooqui M.J. Biol.Chem. 275:40782-40787(2000)
Li H., Leo C., Zhu J., Wu X., O'Neil J., Park E.-J., Chen J.D.Mol. Cell. Biol. 20:1784-1796(2000)

• Western blot analysis of extracts from Hela, K562 and 3T3 cells using HDAC3 Antibody #21660.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting