• Product NameMTERF Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MTERF protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MTERF.
• Target NameMTERF
• Alternative Names    MGC131634; mitochondrial transcription termination factor 1;
• Accession No. Swiss-Prot: Q99551
  NCBI Gene ID: 7978
• UniprotQ99551
• Gene ID7978;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.

Fernandez-Silva P., EMBO J. 16:1066-1079(1997).
Suzuki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 424:157-164(2003).

• Western blot analysis of extracts from Jurkat cells, using MTERF antibody #35106.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using MTERF antibody #35106.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting