• Product NameCytochrome P450 8B1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF ELISA
• Species ReactivityHuman Mouse
• SpecificityThe antibody detects endogenous levels of total Cytochrome P450 8B1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Cytochrome P450 8B1.
• Target NameCytochrome P450 8B1
• Alternative Names    Cytochrome P450; family 8; subfamily B; polypeptide 1; CYP8B1
• Accession No. Swiss-Prot: Q9UNU6
  NCBI Gene ID: 1582
• UniprotQ9UNU6
• Gene ID1582;
• SDS-PAGE MW 58kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500-1:2000
  IHC 1:100-1:300
  IF 1:50-200
  ELISA 1:40000

Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.

Gaafvels M., Genomics 56:184-196(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Muzny D.M., Nature 440:1194-1198(2006)

• Western blot analysis of extracts from 293 cells, using Cytochrome P450 8B1 antibody #35273.
• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using Cytochrome P450 8B1 antibody #35273.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting