• Product NameCalcium Sensing Receptor Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB, ICC/IF, IHC-P
• Species ReactivityHuman, Mouse, Rat
• SpecificityThe antibody detects endogenous levels of total Calcium Sensing Receptor protein.
• Immunogen TypePeptide
• Immunogen DescriptionRecombinant protein encompassing a sequence within the Extracellular domain of human Calcium Sensing Receptor. The exact sequence is proprietary.
• Target NameCalcium Sensing Receptor
• Alternative Names    CAR antibody; EIG8 antibody; FHH antibody; FIH antibody; GPRC2A antibody; HHC antibody; HHC1 antibody; MGC138441 antibody; NSHPT antibody; PCAR1 antibody; CASR antibody; parathyroid cell calcium-sensing receptor antibody; parathyroid Ca(2+)-sensing recept
• Accession No. Swiss-Prot#:P41180;
  NCBI Gene#:846
• UniprotP41180
• Gene ID846;
• SDS-PAGE MW 121kd
• Concentration 0.55mg/ml
• Formulation PBS, 20% Glycerol
• Storage For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

• WB 1:500-1:3000
  ICC/IF 1:100-1:1000
  IHC-P 1:100-1:1000

The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq]

• Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
• Unboiled whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
• Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
• Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor antibody at 1:500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting