• Product NamePLK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PLK1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 267 and 603 of PLK1.
• Target NamePLK1
• Alternative Names    PLK antibody; STPK13 antibody; PLK1 antibody; serine/threonine-protein kinase 13 antibody; polo (Drosophia)-like kinase antibody; polo like kinase antibody; cell cycle regulated protein kinase antibody; serine/threonine-protein kinase PLK1 antibody; PLK-1
• Accession No. Swiss-Prot#:P53350;
  NCBI Gene#:5347
• UniprotP53350
• Gene ID5347;
• SDS-PAGE MW 68kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.

• Sample (30 ug of whole cell lysate)
  A: HeLa
  7.5% SDS PAGE
  #35448 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting