• Product NameNSF Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe antibody detects endogenous levels of total NSF protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 292 of NSF.
• Target NameNSF
• Alternative Names    SKD2 antibody; NSF antibody; vesicle-fusing ATPase antibody; N-ethylmaleimide-sensitive factor-like protein antibody; vesicular-fusion protein NSF antibody; NEM-sensitive fusion protein antibody; N-ethylmaleimide-sensitive fusion protein antibody; N-ethyl
• Accession No. Swiss-Prot#:P46459;
  NCBI Gene#:4905
• UniprotP46459
• Gene ID4905;
• SDS-PAGE MW 83kd
• Concentration 0.27mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:500-1:3000
  

NSF required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

• Sample (50 ug of whole cell lysate)
  A: Mouse brain
  7.5% SDS PAGE
  #35470 diluted at 1:1000
  
• Sample (30 ug of whole cell lysate)
  A: 293T
  7.5% SDS PAGE
  #35470 diluted at 1:1000
• NSF antibody detects NSF protein by Western blot analysis.
  A. 50 ?g rat brain lysate/extract
  7.5 % SDS-PAGE
  #35470 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting