• Product NameTroponin I (cardiac) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by antigen-affinity chromatography.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Troponin I (cardiac) protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fragment corresponding to a region within amino acids 1 and 210 of Troponin I .
• Target NameTroponin I (cardiac)
• Alternative Names    CMD1FF antibody; CMD2A antibody; CMH7 antibody; MGC116817 antibody; RCM1 antibody; TNNC1 antibody; cTnI antibody; TNNI3 antibody; "troponin I; cardiac muscle antibody"; troponin I type 3 (cardiac) antibody
• Accession No. Swiss-Prot#:P19429;
  NCBI Gene#:7137
• UniprotP19429
• Gene ID7137;
• SDS-PAGE MW 24kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:10000
  

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

• Sample (50 ug of whole cell lysate)
  A: mouse heart
  12% SDS PAGE
  #35508 diluted at 1:10000
  
• Sample (30 ug of whole cell lysate)
  A: A431
  B: Molt-4
  12% SDS PAGE
  #35508 diluted at 1:1000
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting