• Product NameACTB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ACTB protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human Actin, beta
• Target NameACTB
• Alternative Names    BRWS1; PS1TP5BP1
• Accession No. Swiss-Prot#: P60709
  NCBI Gene ID: 60
  Gene Accssion: NP_001092
• UniprotP60709
• Gene ID60;
• SDS-PAGE MW 42kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Hela, Jurkat and NIH/3T3 cell, human fetal liver and mouse brain tissue, A431 and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/600 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 7 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting