• Product NameAPIP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total APIP protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human Probable methylthioribulose-1-phosphate dehydratase
• Target NameAPIP
• Alternative Names    APIP2; CGI29; CGI-29; MMRP19; dJ179L10.2
• Accession No. Swiss-Prot#: Q96GX9
  NCBI Gene ID: 51074
  Gene Accssion: BC008440
• UniprotQ96GX9
• Gene ID51074;
• SDS-PAGE MW 27kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury.The protein highly expressed in skeletal muscle and heart and binds to the CARD of Apaf-1 in competition with caspase-9. Exogenous APIP inhibits cytochrome c-induced activation of caspase-3 and caspase-9, and suppresses cell death triggered by mitochondrial apoptotic stimuli through inhibiting the downstream activity of cytochrome c released from mitochondria.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Mouse heart tissue
  Amount of lysate: 60ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #35591 at dilution 1/10.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting