• Product NameGnT-III antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 260 and 495 of Human MGAT3
• Target NameGnT-III
• Accession No. Swiss-Prot:Q09327
  Gene ID:4248
• UniprotQ09327
• Gene ID4248;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 61kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Hep G2
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MGAT3 antibody at 1: 500 dilution.
• Immunofluorescence analysis of methanol-fixed HeLa, using GnT-III antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting