• Product NameAGFG1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total AGFG1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human ArfGAP with FG repeats 1
• Target NameAGFG1
• Alternative Names    HRB; RAB; RIP
• Accession No. Swiss-Prot#: P52594
  NCBI Gene ID: 3267
  Gene Accssion: BC096273
• UniprotP52594
• Gene ID3267;
• SDS-PAGE MW 58kd
• Concentration 3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): A172, Hela and 293T cell, K562 cell and human testis tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/727 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using #36061.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting