• Product NameANKRD54 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ANKRD54 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human ankyrin repeat domain 54
• Target NameANKRD54
• Alternative Names    LIAR
• Accession No. Swiss-Prot#: Q6NXT1
  NCBI Gene ID: 129138
  Gene Accssion: BC014641
• UniprotQ6NXT1
• Gene ID129138;
• SDS-PAGE MW 33kd
• Concentration 2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

Plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. Interacts (via ankyrin repeat region) with LYN (via SH3-domain) in an activation-independent status of LYN By similarity. Forms a multiprotein complex with LYN and HCLS1 By similarity. Interacts with TSN2, VAV1, DBNL AND LASP1.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Mouse testis and human colon cancer tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #36107 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting