• Product NamePGK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PGK2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human phosphoglycerate kinase 2
• Target NamePGK2
• Alternative Names    PGKB; PGKPS; dJ417L20.2
• Accession No. Swiss-Prot#: P07205
  NCBI Gene ID: 5232
  Gene Accssion: BC038843
• UniprotP07205
• Gene ID5232;
• SDS-PAGE MW 45kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): HepG2, 293T, hela and NIH/3T3 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/600 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36193 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting