• Product NameNOL9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NOL9 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human nucleolar protein 9
• Target NameNOL9
• Alternative Names    Grc3; NET6
• Accession No. Swiss-Prot#: Q5SY16
  NCBI Gene ID: 79707
  Gene Accssion: BC112278
• UniprotQ5SY16
• Gene ID79707;
• SDS-PAGE MW 79kd
• Concentration 2.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

Nucleolar protein 9?is a?protein?that in humans is encoded by the?NOL9?gene. Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA.

• Gel: 10%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/1250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting