• Product NameATG9A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ATG9A protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human autophagy related 9A
• Target NameATG9A
• Alternative Names    mATG9; APG9L1; MGD3208
• Accession No. Swiss-Prot#: Q7Z3C6
  NCBI Gene ID: 79065
  Gene Accssion: BC001206
• UniprotQ7Z3C6
• Gene ID79065;
• SDS-PAGE MW 94kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Autophagy-related protein 9A?is a?protein?that in humans is encoded by the?ATG9A?gene. Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes.

• Gel: 6%SDS-PAGE
  Lysate: 40ug 293T cell
  Primary antibody: 1/240 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human brain cancer tissue using #36225 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting