• Product NameASAH1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ASAH1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Target NameASAH1
• Alternative Names    AC; PHP; ASAH; PHP32; ACDase; SMAPME
• Accession No. Swiss-Prot#: Q13510
  NCBI Gene ID: 427
  Gene Accssion: BC016828
• UniprotQ13510
• Gene ID427;
• SDS-PAGE MW 45kd
• Concentration 1.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human lung cancer tissue, human fetal brain and fetal lung tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/850 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #36239 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting