• Product Namep95/NBS1(Phospho-Ser343) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of p95/NBS1 only when phosphorylated at serine 343.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 343 (S-L-S(p)-Q-G) derived from Human p95/NBS1.
• Target Namep95/NBS1
• ModificationPhospho
• Alternative Names    NBN
• Accession No. Swiss-Prot: O60934
  NCBI Protein: NP_002476.2
• UniprotO60934
• Gene ID4683;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 95kd
  Western blotting: 1:500~1:1000
  

Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Hsu HL, et al (2005) Oncogene; 24(31): 4956-64.
Falck J, et al. (2005) Nature; 434(7033): 605-11.
Buscemi G, et al. (2004) Oncogene; 23(46): 7691-700.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101(33): 12130-5.

• Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1(Phospho-Ser343) Antibody #11057.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting