• Product NameAMY2A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB,IHC,ELISA
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total AMY2A protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human amylase, alpha 2A (pancreatic)
• Target NameAMY2A
• Alternative Names    PA; AMY2; AMY2B
• Accession No. Swiss-Prot#: P04746
  NCBI Gene ID: 279
  Gene Accssion: BC007060
• UniprotP04746
• Gene ID279;
• SDS-PAGE MW 58kd
• Concentration 2.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

• Gel: 15%SDS-PAGE
  Lysates (from left to right): Mouse pancreas tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/1400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 second
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #36258 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting