• Product NameTUBB1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 174 and 438 of TUBB1
• Target NameTUBB1
• Accession No. Swiss-Prot:Q9H4B7
  Gene ID:81027
• UniprotQ9H4B7
• Gene ID81027;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 50kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

• Sample(30 ug whole cell lysate)
  A: A431
  B: Hep G2
  10% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TUBB1 antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting