• Product NameRNASE3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RNASE3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human ribonuclease, RNase A family, 3
• Target NameRNASE3
• Alternative Names    ECP; RNS3
• Accession No. Swiss-Prot#: P12724
  NCBI Gene ID: 6037
  Gene Accssion: BC096060
• UniprotP12724
• Gene ID6037;
• SDS-PAGE MW 18kd
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Eosinophil Cationic Protein (ECP) also known as ribonuclease 3 is a basic protein located in the eosinophil primary matrix. In humans, the eosinophil cationic protein is encoded by the RNASE3 gene. ECP is released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body. There are three glycosolated forms of ECP and consequently ECP has a range of molecular weights from 18-22 kDa

• Gel: 10%SDS-PAGE
  Lysate: 40ug U937 cell
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #36432 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting