• Product NameEIF3F Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total EIF3F protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human eukaryotic translation initiation factor 3, subunit F
• Target NameEIF3F
• Alternative Names    EIF3S5; eIF3-p47
• Accession No. Swiss-Prot#: O00303
  NCBI Gene ID: 8665
  Gene Accssion: BC000490
• UniprotO00303
• Gene ID8665;
• SDS-PAGE MW 38kd
• Concentration 1.1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

Eukaryotic translation initiation factor 3 subunit F?is a?protein?that in humans is encoded by the?EIF3F?gene. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse kindey tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using #36438 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting