• Product NameHSD17B12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HSD17B12 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human hydroxysteroid (17-beta) dehydrogenase 12
• Target NameHSD17B12
• Alternative Names    KAR; SDR12C1
• Accession No. Swiss-Prot#: Q53GQ0
  NCBI Gene ID: 51144
  Gene Accssion: BC012043/Q53GQ0
• UniprotQ53GQ0
• Gene ID51144;
• SDS-PAGE MW 34kd
• Concentration 1.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): A375, hela and A172 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/570 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #36529 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting