• Product NameHSD3B1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HSD3B1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the N terminal of human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• Target NameHSD3B1
• Alternative Names    I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
• Accession No. Swiss-Prot#: P14060
  NCBI Gene ID: 3283
  Gene Accssion: BC031999/P14060
• UniprotP14060
• Gene ID3283;
• SDS-PAGE MW 42kd
• Concentration 2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

3 beta-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3β-HSD proteins, designated type I (3β-HSD) and type II (3β-HSD2), are expressed by different genes and function in different areas of the body.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human adrenal gland tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 3 minutes
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36536 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting