• Product NameBUB3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total BUB3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the N terminal of human BUB3 mitotic checkpoint protein
• Target NameBUB3
• Alternative Names    BUB3L; hBUB3
• Accession No. Swiss-Prot#: O43684
  NCBI Gene ID: 9184
  Gene Accssion: BC005138
• UniprotO43684
• Gene ID9184;
• SDS-PAGE MW 37kd
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Jurkat and Hela cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting