• Product NameNOX5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NOX5 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human NADPH oxidase, EF-hand calcium binding domain 5
• Target NameNOX5
• Alternative Names    NOX5A;NADPH oxidase 5;NADPH oxidase
• Accession No. Swiss-Prot#: Q96PH1
  NCBI Gene ID: 79400
  Gene Accssion: BC125098/Q96PH1
• UniprotQ96PH1
• Gene ID79400;
• SDS-PAGE MW 86kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000

This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 293T and hela cell, human testis tissue, skov3, Raji and Jurkat cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting