• Product NameNUMB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total NUMB protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the N terminal of human numb homolog (Drosophila)
• Target NameNUMB
• Alternative Names    S171; C14orf41; c14_5527
• Accession No. Swiss-Prot#: P49757
  NCBI Gene ID: 8650
  Gene Accssion: BC020788/P49757
• UniprotP49757
• Gene ID8650;
• SDS-PAGE MW 71kd
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): K562, hela, skov3, NIH/3T3 and Raji cell, human testis tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting