• Product NameNR4A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NR4A1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human nuclear receptor subfamily 4, group A, member 1
• Target NameNR4A1
• Alternative Names    HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Accession No. Swiss-Prot#: P22736
  NCBI Gene ID: 3164
  Gene Accssion: BC016147
• UniprotP22736
• Gene ID3164;
• SDS-PAGE MW 64kd
• Concentration 1.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  Lysate: 40ug PC3 cell
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting