• Product NameAPAF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total APAF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the C terminal of human Apoptotic protease-activating factor 1
• Target NameAPAF1
• Alternative Names    CED4
• Accession No. Swiss-Prot#: O14727
  NCBI Gene ID: 317
  Gene Accssion: NP_863659
• UniprotO14727
• Gene ID317;
• SDS-PAGE MW 38kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

• Gel: 8%SDS-PAGE
  Lysate: 30ug Hela cell
  Primary antibody: 1/150 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Western blotting analysis using APAF1 Antibody #36718.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting