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HDAC2(Phospho-Ser394) Antibody#11191

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Product Detail

Product NameHDAC2(Phospho-Ser394) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of HDAC2 only when phosphorylated at serine 394.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2.

Target NameHDAC2

ConjugateUnconjugated

Other NamesHD2

Accession NoSwiss-Prot: Q92769
NCBI Protein: NP_001518.2

Uniprot Q92769

Gene ID 3066;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2(Phospho-Ser394) Antibody #11191.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2(Phospho-Ser394) Antibody #11191(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using HDAC2(Phospho-Ser394) Antibody #11191.
Western blot analysis of extracts from HepG2 cells, treated with calf intestinal phosphatase (CIP), using HDAC2 (Phospho-Ser394) Antibody #11191.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes

Tsai SC, et al.(2002)J Biol Chem; 277(35): 31826-33

If you have published an article using product 11191, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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