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CaMKII(Phospho-Thr286) Antibody#11287

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Product Detail

Product NameCaMKII(Phospho-Thr286) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CaMKII only when phosphorylated at threonine 286.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 286 (Q-E-T(p)-V-D) derived from Human CaMKII.

Target NameCaMKII

ConjugateUnconjugated

Other NamesCAMKA

Accession NoSwiss-Prot: Q9UQM7
NCBI Protein: NP_057065.2

Uniprot Q9UQM7

Gene ID 815;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 50kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from 293 cells untreated or treated with PMA using CaMKII(Phospho-Thr286) Antibody #11287.
Western blot analysis of extracts from Rat brain tissue treated with Lambda Phosphotase or calf intestinal phosphatase (CIP),using CaMKII (Phospho-Thr286) Antibody#11287.
Western blotting analysis using CaMKII(Phospho-Thr286) Antibody #11287.

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity

Pak JH, et al. Proc Natl Acad Sci U S A. 2000 Oct 10; 97(21): 11232-11237
Hudmon A, et al. J Cell Biol. Author manuscript; available in PMC 2006 May 7
Miller P, et al. PLoS Biol. 2005 Apr; 3(4): e107
Runyan JD, et al. Learn Mem. 2005 Mar; 12(2): 103-110.

If you have published an article using product 11287, please notify us so that we can cite your literature.

et al,Cardiotoxicity of sorafenib is mediated through elevation of ROS level and CaMKII activity and dysregulation of calcium homoeostasis.In Basic Clin Pharmacol Toxicol on 2020 Feb; by Ma W, Liu M, et al..PMID:31483925 , (2020),
PMID: 31483925
et al,Mechanisms underlying a decrease in KCl-induced contraction after long-term serum-free organ culture of rat isolated mesenteric artery.In J Vet Med Sci on 2014 Jul by Tomoka Morita, Muneyoshi Okada et al..PMID: 24694942 , (2014),
PMID: 24694942
et al,Activation of M3 cholinoceptors attenuates vascular injury after ischaemia/reperfusion by inhibiting the Ca2+/calmodulin鈥恉ependent protein kinase II pathway.In Br J Pharmacol on 2015 Dec by Xing-Zhu Lu, Xue-Yuan Bi et al..PMID: 25953628 , (2015),
PMID: 25953628
et al, HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity.In Sci Rep on 2015 Jun 9 by Thangavel Samikkannu, Kurapati V K Rao et al..PMID: 26057350 , (2014),
PMID: 26057350
et al,Roles of transient receptor potential channel 6 in glucose-induced cardiomyocyte injury. In World J Diabetes on 2022 Apr 15 by Shi-Jun Jiang,et al..PMID:35582666 , (2022),
PMID: 35582666

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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