Product Detail
Product NameVimentin Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total vimentin protein.
Immunogen TypePeptide
Immunogen DescSynthesized peptide derived from Internal of human vimentin.
Target NameVimentin
ConjugateUnconjugated
Other NamesVIME; vimentin;
Accession NoSwiss-Prot: P08670
NCBI Gene ID: 7431
Uniprot
P08670
Gene ID
7431;
Sdspage MW53kd
Concentration1.0mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Vimentin antibody #33541.
Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Vimentin antibody #33541.
Western blot analysis of extracts from 293 cells (Lane 2), using Vimentin antiobdy #33541. The lane on the left is treated with synthesized peptide.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
WB Isaacs, J. Biol. Chem., Oct 1989; 264: 17953 - 17960.
Patric Turowski, Mol. Biol. Cell, Jun 1999; 10: 1997.
Melanie L. Styers, J. Cell Sci., Sep 2006; 119: 3643 - 3654.
Qing-Fen Li, J. Biol. Chem., Nov 2006; 281: 34716 - 34724.
If you have published an article using product 33541, please notify us so that we can cite your literature.
et al,FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis InCell Death DisOn2023 Oct 24byChanghao Wu , Chenglong Zheng et al..PMID:37875474
, (2023),
PMID:
37875474
et al,Monotropein inhibits epithelial-mesenchymal transition in chronic colitis via the mTOR/P70S6K pathway.
, (2025),
PMID:
40041493
Yes
