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Location： Home > Mouse Monoclonal Antibodies > CK5 Antibody

CK5 Antibody#48387

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Product Detail

Product NameCK5 Antibody

Clone No.6D-4D

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, ICC, FC

Species ReactivityHu, Ms, Monkey

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names58 kDa cytokeratin antibody
CK-5 antibody
CK5 antibody
Cytokeratin-5 antibody
Cytokeratin5 antibody
DDD antibody
DDD1 antibody
EBS2 antibody
epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
K2C5_HUMAN antibody
K5 antibody
keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
Keratin 5 antibody
Keratin antibody
keratin complex 2, basic, gene 5 antibody
keratin, type II cytoskeletal 5 antibody
Keratin-5 antibody
Keratin5 antibody
KRT 5 antibody
Krt5 antibody
KRT5A antibody
type II cytoskeletal 5 antibody
Type-II keratin Kb5 antibody

Accession NoSwiss-Prot#:P13647

Uniprot P13647

Gene ID 3852;

Calculated MW62 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of CK5 on different cells lysates using anti-CK5 antibody at 1/1,000 dilution. Positive control��
Line1: A431
Line2: 3T3-L1
Line3: COS7
Line4: MCF-7
Line5: SK-Br-3
Line6: Hela
Line7: Lncap
Line8: HepG2
Western blot analysis of CK5 on human CK5 recombinant protein using anti-CK5 antibody at 1/1,000 dilution.
Western blot analysis of CK5 on HEK293 (1) and CK5-hIgGFc transfected HEK293 (2) cell lysate using anti-CK5 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- CK5 antibody. Counter stained with hematoxylin.
ICC staining CK5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with CK5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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