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Histone H3.1 Antibody#48540

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Product Detail

Product NameHistone H3.1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationPeptide affinity purified.

ApplicationsWB,ICC,IHC

Species ReactivityHu, Ms

Immunogen DescSynthetic peptide of N-terminal human Histone H3.1.

ConjugateUnconjugated

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Calculated MW15.4 kDa

Formulation1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Histone H3.1 on F9 cell lysate using anti-Histone H3.1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H3.1 antibody. Counter stained with hematoxylin.
ICC staining Histone H3.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Histone H3.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

If you have published an article using product 48540, please notify us so that we can cite your literature.

et al,Guanylate Binding Protein 1-Mediated Interaction of T Cell Antigen Receptor Signaling with the Cytoskeleton , (2014),
PMID: 24337748

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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